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Archive Number 20081012.3234
Published Date 12-OCT-2008
Subject PRO/AH/EDR> Undiagnosed fatalities - S. Africa ex Zambia (07): arenavirus
UNDIAGNOSED FATALITIES - SOUTH AFRICA ex ZAMBIA (07): ARENAVIRUS
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Date: 12 Oct 2008
Source: The Mercury [edited]
<http://www.themercury.co.za/?fSectionId=3535&fArticleId=nw20081012152218513C975048>


The mystery viral hemorrhagic fever which killed 3 people in South 
Africa has been provisionally identified as an arenavirus, the 
National Institute for Communicable Diseases [NICD] and the 
Department of Health said on Sunday [12 Oct 2008].

"The causative agent of the disease may be a rodent-born arenavirus 
related to the Lassa fever virus of West Africa," NICD's Dr Lucille 
Blumberg said at the Charlotte Maxexe Johannesburg Academic Hospital.

She said tests done by the NICD and the Centers for Disease Control 
[CDC] in Atlanta, USA indicated that the disease seemed to be a kind 
of arenavirus. The World Health Organisation has also been providing 
technical assistance.

Arenaviruses cause chronic infections in multimammatic mice, a kind 
of wild mouse, which excrete the virus in their urine, which can then 
contaminate human food or house dust.

Viruses similar to the Lassa fever virus have been found in rodents 
in Africa, but other than in West Africa have not been found to cause 
diseases in humans.

She said there was no indication arenaviruses that could cause 
disease in humans were present in South African rodents.

Blumberg said further tests still needed to be done. "It needs to be 
determined whether it is a previously unrecognized member of the 
arenaviruses and what its distribution is," she said.

The NICD's Robert Swanepoel said there were viruses of this family in 
Southern Africa but that this could be an undiscovered kind. "Not 
every country has been thoroughly searched," he said. He said the 
kind of rodents that carried the virus were not generally found in 
urban areas. "They are out there but attracted (to human dwellings) 
if there is inadequate waste disposal." Crop disposal and animal feed 
also sometimes attracted them, he said. He said the kinds of viruses 
could range from causing mild fevers to being lethal. There were only 
3 cases to work with for the kind of arenavirus now discovered, but 
"it looks like it is very lethal," he said.

Head of the NICD's Special Pathogens Unit, Dr Janusz T Paweska, said 
the arenavirus diagnosis came about after a number of tests. 
[Biopsies] conducted on the last 2 victims in which infected tissues, 
skin, liver and muscles were tested were critically important in 
making a diagnosis. A blood sample obtained in Zambia from the 1st 
victim also confirmed test results.

He said doctors were now waiting for the virus to grow in cell 
culture to conduct further tests to identify what strain it was.

Gauteng health MEC [Brian Hlongwa?] said the 1st victim of the virus 
was [a] 36-year-old [woman], who was airlifted from Zambia to the 
Morningside Medi Clinic in Sandton on [12 Sep 2008] in a critical condition.

She is known to have lived in a smallholding on the outskirts of 
Lusaka where she kept 3 horses, although the exact point of 
contamination has never been discovered.

She fell ill on [8 Sep 2008] and was treated in 3 different hospitals 
in Lusaka. Once in South Africa, she was treated for tick bite fever 
and other potential infections but died 2 days later. She was not 
tested for viral hemorrhagic fever.

On [27 Sep 2008], a Zambian paramedic who accompanied her into the 
country was admitted to the hospital with similar flu-like symptoms, 
fever and a skin rash, and viral hemorrhagic fever was queried.

He developed diarrhea, severe headaches, nausea and vomiting, and 
although he initially seemed to respond to treatment, he died on [2 
Oct 2008] at the clinic.

A 3rd victim of the virus was a nurse from Morningside Medi-Clinic 
who attended [the 1st case]. She became ill with fever 18 days after 
[the woman from Zambia] was admitted to the hospital, and consulted a 
general practitioner, receiving intravenous therapy. She was then 
referred to Robinson Hospital in Randfontein and was later 
transferred due to a bedding shortage to Sir Albert Clinic. There, 
she was treated for a suspected case of meningitis. Her condition 
deteriorated, and she died last Sunday [5 Oct 2008].

A 4th person, a [37-year-old female] contract cleaner working at 
Morningside Medi-clinic, died in Charlotte Maxeke Academic hospital. 
Earlier this week, the health department said her death was not 
related to viral hemorrhagic fever.

On Sunday [12 Oct 2008?], Blumberg said a female nurse and a male 
paramedic were currently in isolation after they were in contact with 
the deceased.

The paramedic had contact with [the 1st case, the female from 
Zambia], and after developing flu-like symptoms and a fever was 
admitted to Flora clinic. He was subsequently transferred to 
Morningside Medi-clinic and diagnosed with kidney stones. On Sunday 
[12 Oct 2008], Blumberg said it was "less likely" he had the virus.

The 2nd person in isolation is a nurse who had contact with a 
paramedic that died [the 2nd patient, the male paramedic that 
accompanied the 1st patient to South Africa]. She has developed 
symptoms similar to the 3 deceased and is receiving anti-viral 
medication called ribavirin. The department of health said she was 
presently stable. Blumberg said she is "highly suspect" to have 
contracted the virus and could not say further how her condition was 
likely to progress.

This week, 3 other people who had been hospitalized after contact 
with the deceased were discharged. On Friday morning [10 Oct 2008], 
the 11-year-old son of the nursing sister who died [case number 3] 
and his 23-year-old nanny were discharged [presumably discharged from 
quarantine].

A cleaning supervisor at Morningside Medi-Clinic who had been 
admitted to the Chris Hani Baragwanath Hospital on Monday [6 Oct 
2008] with symptoms of viral hemorrhagic fever was also released. On 
Sunday [12 Oct 2008], Hlongwa said she was currently "well."

All 3 continued to be monitored as part of the disease surveillance 
system currently tracking 151 people who had contact with the deceased.

Blumberg said arenaviruses could cause a disease which spreads from 
human to human through contact fluids. In hospital settings, special 
precautions were needed when nursing patients.

People in contact with those who have contracted the virus must be 
monitored for 21 days following their last contact with the patient. 
Their body temperature is monitored, and those who develop fever or 
illness are admitted to an isolation ward in the hospital.

Blumberg said there was a drug which showed promising results in 
treating patients if their illness is recognized early.

Those who have been in contact with patients but are well do not 
spread infection.

On Sunday [12 Oct 2008], Gauteng health MEC Brian Hlongwa said the 
diagnosis of the virus was a step forward. "We are now a step further 
because we know specifically what we are dealing with." However, it 
was still vital to conduct more tests to find out what kind of 
arenavirus it was, he said.

Director General Thami Mseleku cautioned South Africans not to now 
fear that every mouse that came their way contained the virus.

Since the virus 1st broke out, medical officials have been at pains 
to emphasize that the general public is not at risk, as only people 
who were in direct contact with the bodily fluids of a person who had 
a confirmed case of the virus could be infected.

--
Communicated by:
HealthMap Alert via
ProMED-mail <promed@promedmail.org>

[According to the above newswire, there has been a "preliminary" 
identification of an arenavirus in specimens from the initial 3 
fatalities felt to be associated in this cluster. Apparently, the 
virus was identified in infected tissue of the 2nd and 3rd case (the 
paramedic accompanying the 1st case during the medical transfer from 
Zambia to South Africa and a nursing sister who cared for the 1st 
case while hospitalized in South Africa) and further identified 
through testing of blood from the original case (the 36-year-old 
woman from Zambia.) We await further information on this from official sources.

Former viral disease moderator Prof. Charles H. Calisher had the 
following to add: "There are at least 23 recognized arenaviruses 
(family _Arenaviridae_, genus _Arenavirus_).  The conventional 
phylogeny of these viruses distinguishes "Old World" from "New World" 
arenaviruses, i.e., based on geographic distribution.  As are the 
hantaviruses, each arenavirus is principally associated with rodents 
of a single or a very few species, thus the basis for their 
geographic distributions.  An unusual exception may be Tacaribe 
virus, which has been isolated from bats and mosquitoes.  Most New 
World arenaviruses are not known to cause human illnesses but others 
cause severe illnesses: Machupo virus (Bolivian hemorrhagic fever), 
Junin virus (Argentine hemorrhagic fever), Guanarito virus 
(Venezuelan hemorrhagic fever), Sabia virus (Brazilian hemorrhagic 
fever), and Chapare virus.

Lassa virus has been shown to cause severe, often fatal, hemorrhagic 
fevers in human in a wide area of West Africa.  It is now recognized 
that this virus causes numerous illnesses each year.  It will be 
enlightening when we have news of the identity of the viruses causing 
these illnesses in Zambia, and now in South Africa. The 
investigations are in the hands of the world's most competent people 
for such studies, Dr. Robert Swanepoel of South Africa's National 
Institute for Communicable Diseases and collaborating investigators 
at the U.S. Centers for Disease Control and Prevention.  Therefore, 
definitive information regarding whether this is a hitherto 
unrecognized arenavirus or a variant of a recognized arenavirus 
should be forthcoming relatively soon.  Meanwhile, clinical practices 
to control further spread should suffice."

If the etiologic agent is in fact an arenavirus, ribavirin is 
presently used for the treatment of Lassa fever. This moderator would 
like to thank Prof. C.J. Peters, a leading authority on 
arenaviruses, for kindly providing the references listed below on 
treatment modalities used for arenavirus infection.

References.
1. Barry, M., M. Russi, L. Armstrong, D. Geller, R. Tesh, L. Dembry, 
J. P. Gonzalez, A. S. Khan, and C. J. Peters. 1995. Brief report: 
treatment of a laboratory-acquired Sabia virus infection.[see 
comment]. New England Journal of Medicine 333:294-296.
2. Enria, D. A., A. M. Briggiler, S. Levis, D. Vallejos, J. I. 
Maiztegui, and P. G. Canonico. 1987. Tolerance and antiviral effect 
of ribavirin in patients with Argentine hemorrhagic fever. Antiviral 
Research 7:353-359.
3. Jahrling, P. B., R. A. Hesse, G. A. Eddy, K. M. Johnson, R. T. 
Callis, and E. L. Stephen. 1980. Lassa virus infection of rhesus 
monkeys: pathogenesis and treatment with ribavirin. Journal of 
Infectious Diseases 141:580-589.
4. Kilgore, P. E., T. G. Ksiazek, P. E. Rollin, J. N. Mills, M. R. 
Villagra, M. J. Montenegro, M. A. Costales, L. C. Paredes, and C. J. 
Peters. 1997. Treatment of Bolivian hemorrhagic fever with 
intravenous ribavirin. Clinical Infectious Diseases 24:718-722.
5. McCormick, J. B., I. J. King, P. A. Webb, C. L. Scribner, R. B. 
Craven, K. M. Johnson, L. H. Elliott, and R. Belmont-Williams. 1986. 
Lassa fever. Effective therapy with ribavirin. New England Journal of 
Medicine 314:20-26.
6. Peters CJ. 2002. Arenaviruses, p. 949-969. In Richman DD, Whitely 
RJ, and Hayden FG (eds.), Clinical Virology. ASM Press, Washington, DC.
7. Peters CJ, Zaki SR, and Rollin PE. 1997. Viral hemorrhagic fevers, 
p. 10.l-10.26. In Mandell GL and Fekety R (eds.), Atlas of Infectious 
Diseases, Vol. 8. Current Medicine, Philadelphia.
- Mod.MPP]

[see also:
Undiagnosed fatalities - South Africa ex Zambia (06): WHO 20081010.3211
Undiagnosed fatalities - South Africa ex Zambia (05) 20081008.3192
Undiagnosed fatalities - South Africa ex Zambia (04) 20081008.3188
Undiagnosed fatalities - South Africa ex Zambia (03) 20081007.3178
Undiagnosed fatalities - South Africa ex Zambia (02) 20081006.3157
Undiagnosed fatalities - South Africa ex Zambia: RFI 20081005.3139
Human lymphocytic choriomeningitis virus - USA (03) 20080726.2276
Human lymphocytic choriomeningitis virus - USA (02) 20080516.1635
Human lymphocytic choriomeningitis virus - USA: organ donation 20080412.1339
Lassa fever - Nigeria (02) 20080611.1847
Lassa fever - Nigeria: (Ebonyi) 20080323.1100
Arenavirus hemorrhagic fever - Bolivia: (Chapare), new arenavirus 20080418.1387
2007
----
Arenavirus, organ transplants - Australia (VIC)(02) 20070430.1402
Arenavirus, organ transplants - Australia (VIC) 20070423.1325
Bolivian hemorrhagic fever - Bolivia (Beni)(02) 20070315.0914
2003
----
Arenaviruses and rodent taxonomy (02) 20030412.0891
Arenaviruses and rodent taxonomy 20030411.0879
Lassa fever - Sierra Leone 20030409.0863
Lassa fever, imported - UK ex Sierra Leone 20030210.0360
2002
----
Venezuelan hemorrhagic fever - Venezuela (02) 20020204.3479
Venezuelan hemorrhagic fever - Venezuela 20020203.3467
2001
----
Argentinean hem. fever - Argentina (Buenos Aires Prov.) 20010404.0675
2000
----
Arenavirus infection, human USA (CA): Confirmed (02) 20000813.1344
Arenavirus infections, human - USA (CA): Confirmed 20000804.1301
Arenavirus infection, human - USA (Calif.): comment (02) 20000708.1137
Arenavirus infection, human - USA (Calif.): comment 20000704.1110
Arenavirus infection, human - USA (California) 20000703.1104
Lassa virus, seroprevalence 20000119.0080
1996
----
List of recognized arenaviruses 19961218.2106]
...............................................mpp/msp/mpp/dk

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