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Archive Number 20081013.3241
Published Date 13-OCT-2008
Subject PRO/AH/EDR> Undiagnosed fatalities - S. Africa ex Zambia (08): arenavirus
UNDIAGNOSED FATALITIES - SOUTH AFRICA EX ZAMBIA (08): ARENAVIRUS IDENTIFIED
***************************************************************************
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In this update:
[1] South Africa - National Institute for Communicable Diseases (NCID)
[2] WHO
[3] South Africa Government Communication and Information System
[4} Newswire

****
[1] South Africa - National Institute for Communicable Diseases (NCID)
Date: Mon 13 Oct 2008
From: Liz Millington <lizm@nicd.ac.za>


Outbreak of an acute disease, ex Zambia
---------------------------------------
The results of tests conducted at the Centers for Disease Control in 
Atlanta (CDC), USA, and at the National Institute for Communicable 
Diseases of the National Health Laboratory Service in Johannesburg, 
provide preliminary evidence that the causative agent of the disease 
that has resulted in the recent deaths of 3 people in hospitals in 
Johannesburg, is a rodent-borne arenavirus related to the Lassa fever 
virus of West Africa. Further tests, to confirm the diagnosis by 
growing the virus in culture and to characterize it further, are in 
progress. It needs to be determined whether it is a previously 
unrecognized member of the arenaviruses, and what its distribution 
and reservoir host are.

Arenaviruses cause chronic infection in wild rodents (multimammate 
mice) with excretion of virus in urine, which can contaminate human 
food or house dust. Arenaviruses have been found in southern African 
rodents in the past, but there has been no previous association with 
human disease. The virus associated with the present outbreak may 
prove to be a new member of the group.

In the current outbreak there have been 3 deaths, the index case 
ex-Zambia and 2 persons who acquired disease after close contact in 
the nosocomial setting. A 4th patient, a nurse, who had close contact 
with the 2nd patient, has developed a febrile illness and 
thrombocytopenia, and has tested positive by PCR [polymerase chain 
reaction] for arenavirus. She is currently being treated with 
ribavirin. The efficacy of ribavirin has been documented in Lassa 
fever, but is unknown for the current virus.

The incubation period for cases in the present cluster ranges from 
7-13 days. There is a prodromal illness of about 7 days with myalgia, 
headache, diarrhoea, and a severe pharyngitis. This is followed by a 
more severe illness with moderate thrombocytopenia but no bleeding. 
Hepatic dysfunction with raised transaminases has typically occurred 
late in the course of disease.

[Source: Centers for Disease Control and Prevention, Atlanta, USA
National Institute for Communicable Diseases of the National Health 
Laboratory Service, Johannesburg, South Africa
Department of Pathology, University of the Witwatersrand, 
Johannesburg, South Africa]

--
Communicated by:
Liz Millington on behalf of
Dr Lucille Blumberg
National Institute for Communicable Diseases
1 Modderfontein Road
Sandringham 2131
South Africa
<lucilleb@nicd.ac.za>

******
[2] WHO
Date: Mon 13 Oct  2008
Source: World Health Organisation (WHO), EPR, Disease Outbreak News [edited]
<http://www.who.int/csr/don/2008_10_13/en/index.html>


New virus from family _Arenaviridae_ in South Africa and Zambia - Update
----------------------------------------------------------------------------------------------
The results of tests conducted at the Special Pathogens Unit, 
National Institute for Communicable Diseases (NICD) of the National 
Health Laboratory Service in Johannesburg, and at the Special 
Pathogens and Infectious Disease Pathology branches of the Centers 
for Disease Control in Atlanta, USA, provide preliminary evidence 
that the causative agent of the disease which has resulted in the 
recent deaths of 3 people from Zambia and South Africa, is a virus 
from the family _Arenaviridae_.

Analysis continues at the NICD and CDC in order to characterize this 
virus more fully. CDC and NICD are technical partners in the Global 
Outbreak Alert and Response Network (GOARN).

Meanwhile, a new case has been confirmed by PCR in South Africa. A 
nurse who had close contact with an earlier case has become ill, and 
has been admitted to hospital. Contacts have been identified and are 
being followed-up.

WHO and its GOARN partners continue to support the Ministries of 
Health of the two countries in various facets of the outbreak 
investigation, including laboratory diagnosis, investigations, active 
case finding and follow-up of contacts.

--
Communicated by:
ProMED-mail Rapporteur Marianne Hopp

******
[3] South Africa Government Communication and Information System
Date: Mon 13 Oct 2008
Source: BuaNews online, compiled by the Government Communication and 
Information System [edited]
<http://www.buanews.gov.za/news/08/08101311151006>


Unknown illness identified as Arenavirus
----------------------------------------
The virus, which has caused the death of 3 people, has been 
provisionally identified as the rodent-borne arenavirus. The 
arenavirus, related to Lassa fever virus of West Africa, causes 
chronic infections in multimammate mice. Infected mice's excretion 
contains the virus, which can contaminate human food or house dust.

A joint statement by the National Institute for Communicable Disease 
(NICD) and the Department of Health explained that the arenavirus 
[infection] is a disease spread from human to human through the 
contact of body fluids. The finding follows blood samples being sent 
to Atlanta, in the United States to determine the cause of the deaths 
of 3 people who had been suspected of contracting [a] viral 
haemorrhagic fever. The virus is similar to Lassa fever [virus], the 
department said. It has previously been found in rodents elsewhere in 
Africa, but has not been found to cause disease in humans other than 
in West Africa.

Further tests are needed to confirm the diagnosis by growing the 
virus in culture. "It needs to be determined whether it is a 
previously unrecognised member of the arenaviruses [the family 
_Arenaviridae_], and what its distribution is. There is no indication 
as yet that arenaviruses which cause disease in humans are present in 
South African rodents," the NICD said.

The 1st victim, who had to be flown in from Zambia in a critical 
condition, was admitted to the Morningside Medi-Clinic in mid 
September [2008]. She died 2 days later. About 2 weeks later, the 
paramedic who had flown in with the 1st victim, was admitted at the 
same clinic presenting the same symptoms. A nurse, died shortly 
afterwards. According to certain reports the nurse's family has been 
given a go-ahead to continue with the funeral arrangements as her 
bedroom had been cordoned off by health officials. A cleaner at the 
Morningside Medi-Clinic, who also died last weekend, has since been 
ruled out as a possible victim of the virus. Meanwhile the Gauteng 
Health Department has confirmed that the 3 other patients, including 
nurse's female supervisor, who had been under observation for showing 
symptoms of the virus have been discharged.

However, departmental spokesperson Phumelele Kaunda said there were 2 
contacts that were still under active surveillance after being 
admitted for observation. The one patient is a paramedic who had 
contact with the 1st patient and developed fever and flu-like 
symptoms. He was admitted initially in Flora Clinic and then 
transferred to Morningside Medi-Clinic with a diagnosis of kidney 
stones. The other patient is a nurse who attended to the 2nd patient 
and developed signs and symptoms similar to the 1st 3 patients. She 
is being treated in isolation and received the anti-viral medication, 
ribavirin. The patient is presently stable.

Gauteng Health MEC [member of the executive council] Brian Hlongwa 
meanwhile has sent condolences to the families of those that were 
killed by the viral infection, particularly families of health 
professionals who died in the line of duty. "This illustrates the 
dedication of our health professionals and the need of society to 
respect and honour the work that they do," said MEC Hlongwa. He also 
thanked the NICD, the National Health Laboratory Service, Centre for 
Disease Control in Atlanta, and the World Health Organisation for 
ensuring that the results were made available soon.

[Byline: Luyanda Makapela]

--
Communicated by:
ProMED-mail Rapporteur Brent Barrett

******
[4] Newswire
Date: Sun 12 Oct 2008
Source: News24, South Africa, South African Press Association (SAPA) 
report [edited]
<http://www.news24.com/News24/South_Africa/News/0,,2-7-1442_2408685,00.html>


Virus identified - nurse ill
----------------------------
The mystery viral haemorrhagic fever, which killed 3 people in South 
Africa, has been provisionally identified as an arenavirus, the 
National Institute for Communicable Diseases (NICD) and the 
Department of Health said on Sunday [12 Oct 2008]. "The causative 
agent of the disease... may be a rodent-borne arenavirus related to 
the Lassa fever virus of West Africa," NICD's Dr Lucille Blumberg 
told reporters at the Charlotte Maxeke Johannesburg Academic 
Hospital. She said tests done by the NICD and the Centres for Disease 
Control in Atlanta, US, indicated that the disease seemed to be a 
kind of an arenavirus. The World Health Organisation (WHO) has also 
been providing technical assistance in identifying the virus.

Arenaviruses cause chronic infections in multimammate mice -- a kind 
of wild mouse -- that excrete the virus in their urine, which can 
then contaminate human food or house dust. Viruses similar to the 
Lassa fever virus have been found in rodents in Africa, but other 
than in West Africa have not been found to cause diseases in humans. 
She said there was no indication that arenaviruses, which could cause 
disease in humans, were present in South African rodents. "It needs 
to be determined whether it is a previously unrecognised member of 
the arenaviruses and what its distribution is," she said.

The NICD's Robert Swanepoel said there were viruses of this family in 
Southern Africa, but that this could be an undiscovered kind. He said 
the kind of rodents who carried the virus was not generally found in 
urban areas. "They are out there, but attracted [to human dwellings] 
if there is inadequate waste disposal."

Crops and animal feed also sometimes attracted them, he said. He said 
the kinds of viruses could range from causing mild fevers to being 
lethal. There were only 3 cases to go on for the kind of arenavirus 
now discovered, but "it looks like it is very lethal," he said.

Head of the NICD's Special Pathogens Unit, Dr Janusz T Paweska, said 
the arenavirus diagnosis came about after a number of tests. Biopsies 
conducted on the last 2 victims where infected tissues, skin, liver, 
and muscles were tested were critically important in being able to 
make a diagnosis. A blood sample obtained in Zambia from the 1st 
victim also confirmed test results. He said doctors were now waiting 
for the virus to grow in cell culture to conduct further tests to 
identify what strain it was.

Gauteng Health MEC Brian Hlongwa said the 1st victim of the virus was 
36-year-old woman, who was airlifted from Zambia to the Morningside 
Medi-Clinic in Sandton on [12 Sep 2008] in a critical condition. She 
is known to have lived in a smallholding on the outskirts of Lusaka 
where she kept 3 horses and other animals, although the exact point 
of contamination has never been discovered. She fell ill on 8 Sep 
2008 and was treated in 3 different hospitals in Lusaka. Once in 
South Africa she was treated for tick bite fever and other potential 
infections, but died 2 days later.

On [27 Sep 2008] a Zambian paramedic who accompanied her into the 
country was admitted into the hospital with similar flu-like 
symptoms, fever, and a skin rash. In his case, viral haemorrhagic 
fever was queried. He developed diarrhoea, severe headaches, nausea, 
and vomiting and although he initially seemed to respond to 
treatment, died on [2 Oct 2008] at the clinic.

A 3rd victim of the virus was a nurse from Morningside Medi-Clinic 
who attended to index case. She became ill with fever 18 days after 
the [index case] was admitted to the hospital and consulted a general 
practitioner, receiving intravenous therapy. She was then referred to 
Robinson Hospital in Randfontein and later transferred due to a 
bedding shortage to Sir Albert Clinic. Here she was treated for a 
suspected case of meningitis. Her condition deteriorated and she died 
last Sunday [5 Oct 2008].

A 4th person, a contract cleaner working at Morningside Medi-clinic 
Maria Mokubung, 37, died in Charlotte Maxeke. Earlier this week the 
Health Department said her death was not related to viral 
haemorrhagic fever. On Sunday [12 Oct 2008], Blumberg said a female 
nurse and a male paramedic were currently in isolation after they 
were in contact with the deceased. The paramedic had contact with 
[the index case] and after developing flu-like symptoms and a fever 
was admitted to Flora clinic. He was subsequently transferred to 
Morningside Medi-Clinic and diagnosed with kidney stones. On Sunday 
Blumberg said it was "less likely" he had the virus.

The 2nd person in isolation is a nurse who had contact with the 
paramedic that died. She has developed symptoms similar to the 3 
deceased and is receiving anti-viral medication called ribavirin. The 
department of health said she was presently stable. Asked whether she 
could have contracted the virus, Blumberg said her condition was 
"highly suspect." She said she could not say further how her 
condition was likely to progress.

This week [week of 6 Oct 2008] 3 other people who had been 
hospitalised after contact with the deceased were discharged. On 
Friday morning [10 Oct 2008] the 11 year-old son of the nursing 
sister who died and his 23-year-old nanny were discharged. A cleaning 
supervisor at Morningside Medi-Clinic who had been admitted to the 
Chris Hani Baragwanath Hospital on Monday [6 Oct 2008] with symptoms 
of viral haemorrhagic fever was also released. All 3 continued to be 
monitored as part of the disease surveillance system currently 
tracking 151 people who had contact with the deceased.

Blumberg said arenaviruses could cause a disease, which spreads from 
human to human through contact fluid. In hospital settings, special 
precautions were needed when nursing patients. People in contact with 
those who have contracted the virus must be monitored for 21 days 
following their last contact with the patient. Their body temperature 
is monitored and those who develop fever or illness are admitted to 
an isolation ward in the hospital. Blumberg said there was a drug, 
which showed promising results in treating patients if their illness 
was recognised early. Those who have been in contact with patients 
but are well do not spread infection.

On Sunday [12 Oct 2008], Hlongwa said the diagnosis of the virus was 
a step forward. However, it was still vital to conduct more tests to 
find out what kind of arenavirus it was, he said. Health department 
director-general Thami Mseleku cautioned South Africans not to now 
fear that every mouse that came their way contained the virus. Since 
the virus first broke out, medical officials have been at pains to 
emphasise that the general public is not at risk as only people who 
were in direct contact with the bodily fluids of a person who had a 
confirmed case of the virus could be infected.

--
Communicated by:
ProMED-mail and a similar report ProMED-mail Rapporteur Mary Marshall
<promed@promedmail.org>

[These 3 accounts confirm that an unidentified arenavirus was 
responsible for the deaths of the 3 patients in South Africa. 
Contrary to a previous account a sample from the index case was 
available and has tested positive for the unidentified arenavirus. 
Also a 4th critically ill surviving patient (who nursed the index 
case) in South Africa has tested positive for the same virus. None of 
the other suspected cases have been confirmed so far. As the virus 
has not yet been propagated in cell culture it must be presumed that 
the identification is based on PCR assay.

The family _Arenaviridae_ is split into Old and New world groups, 
which are only distantly related. There are 5 species of Old World 
viruses -- Ippy virus, Lassa virus, lymphocytic choriomeningitis 
virus (LCMV), Mobala virus, and Mopeia virus. The cosmopolitan LCMV 
is a mouse virus, whereas Lass virus and Mopeia virus share a common 
rodent host, species of the multimammate mouse (genus _Mastomys__). A 
photograph of a multimammate mouse can be seen at 
<http://animal.nibio.go.jp/ypc_img_1413_600.jpg>.

Lassa virus is present in west and central Africa and has been 
associated with disease in humans and other primates, whereas Mopeia 
virus is present further south and not known to be associated with 
disease in humans or primates so far. - Mod.CP]

[see also:
Undiagnosed fatalities - S. Africa ex Zambia (07): arenavirus 20081012.3234
Undiagnosed fatalities - South Africa ex Zambia (06): WHO 20081010.3211
Undiagnosed fatalities - South Africa ex Zambia (05) 20081008.3192
Undiagnosed fatalities - South Africa ex Zambia (04) 20081008.3188
Undiagnosed fatalities - South Africa ex Zambia (03) 20081007.3178
Undiagnosed fatalities - South Africa ex Zambia (02) 20081006.3157
Undiagnosed fatalities - South Africa ex Zambia: RFI 20081005.3139
Human lymphocytic choriomeningitis virus - USA (03) 20080726.2276
Human lymphocytic choriomeningitis virus - USA (02) 20080516.1635
Human lymphocytic choriomeningitis virus - USA: organ donation 20080412.1339
Lassa fever - Nigeria (02) 20080611.1847
Lassa fever - Nigeria: (Ebonyi) 20080323.1100
2007
----
Arenavirus, organ transplants - Australia (VIC)(02) 20070430.1402
Arenavirus, organ transplants - Australia (VIC) 20070423.1325
2003
----
Arenaviruses and rodent taxonomy (02) 20030412.0891
Arenaviruses and rodent taxonomy 20030411.0879
Lassa fever - Sierra Leone 20030409.0863
Lassa fever, imported - UK ex Sierra Leone 20030210.0360
2000
----
Arenavirus infection, human USA (CA): Confirmed (02) 20000813.1344
Arenavirus infections, human - USA (CA): Confirmed 20000804.1301
Arenavirus infection, human - USA (Calif.): comment (02) 20000708.1137
Arenavirus infection, human - USA (Calif.): comment 20000704.1110
Arenavirus infection, human - USA (California) 20000703.1104
Lassa virus, seroprevalence 20000119.0080
1996
----
List of recognized arenaviruses 19961218.2106]
...................................mpp/cp/mj/mpp/cp/mpp

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