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Archive Number 20080805.2400
Published Date 05-AUG-2008
Subject PRO/AH/EDR> Undiagnosed hem. disease - China (02): (SD), anaplasmosis
UNDIAGNOSED HEMORRHAGIC DISEASE - CHINA (02): (SHANDONG), ANAPLASMOSIS
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Date: Tue 5 Aug 2008
From: Hans Troedsson <who.chn@wpro.who.int>


The WHO [World Health Organization] China Office has had ongoing 
communications with the Ministry of Health China regarding 
"Undiagnosed hemorrhagic disease - China: (Shandong) RFI Archive 
Number 20080727.2300. The Chinese experts have completed an 
investigation into the incident. Four cases of human granulocytic 
anaplasmosis were identified in Wanjiakou village in Shandong 
province. The diagnosis was laboratory confirmed by PCR [polymerase 
chain reaction] and sequencing analysis.

To our knowledge, the 1st case was a 43-year-old male farmer 
hospitalized on 18 Jun 2008 (with fever, leucopenia, and 
thrombocytopenia) and who died on 23 Jun 2008 despite treatment. The 
father of this patient was also identified with the disease, had 
onset of symptoms on 1 Jul 2008, and died on 10 Jul 2008. Two other 
cases were found in the same village on 2 and 5 Jul 2008. They 
recovered after being treated and were discharged from hospital on 13 
and 14 Jul 2008 respectively. Active case finding has not identified 
additional cases.

Anaplasmosis is a tick-borne disease, not known to spread by 
human-to-human transmission. China has lately strengthened its 
prevention and control measures as well as improved diagnosis and 
treatment guidelines for tick-borne diseases. The disease is not 
notifiable in China but has been recognized before in China as well 
as in other countries including the USA, several European countries, 
and recently in Canada.

These are sporadic cases of anaplasmosis. The disease can be treated 
effectively [with] antibiotics. These cases of anaplasmosis do not 
pose an outbreak or epidemic threat. Further information may be 
provided by the Ministry of Health upon request.

--
Dr Hans Troedsson
World Health Organization Representative to China
<who.chn@wpro.who.int>

[ProMED-mail thanks Dr Troedsson for his response to our request for 
information. In the original posting, the following is reported:

"A man suddenly died from an unidentified disease in Wanjiakou 
Village, Xiaoguan Town, Wendeng City, Shandong Province. His entire 
body turned dark purple, and he bled from his mouth, nostrils, ears, 
and eyes just as he died.

Shortly after the man died, 2 other men who [had] been in contact 
with him, died showing the same symptoms. Villagers who had left the 
village to work said "3 people died 10 days ago."

It may well be the case that this initial description is inaccurate. 
Human granulocytic anaplasmosis (HGA) has a quite low case fatality 
rate especially in seemingly otherwise healthy people. A mortality 
rate of 2 out of 4 cases (50 percent) would be unusual for HGA as 
would be an apparent cluster.

Mortality seems to be related to multiorgan failure and although a 
low platelet count may cause bleeding, the circumstances described in 
the initial posting are likely not due to a low platelet count. - Mod.LL]

[Background information on HGA in China is available on the Global 
Infectious Disease and Epidemiology Network (GIDEON) website 
(<http://www.gideononline.com>).

"Seropositive ruminants have been documented in Xinjiang Uygur 
Autonomous Region area. (see ref 1 below)
- Seropositive rodents have been documented in Jilin Province: 
Japanese field mouse (_Apodemus peninsulae_), Black-striped field 
mouse (_A. agrarius_) and Siberian chipmunk (_Tamias sibiricu_). (see 
ref 2 below)

"Ehrlichial DNA (Anaplasma phagocytophilum) has been detected in 
_Ixodes persulcatus_ ticks from the Northeastern (ie, Lyme disease) 
region - Inner Mongolia and Heilongjiang Province. (see refs 3 and 4 below)
- Infected I. persulcatus and Dermacentor silvarum have been detected 
in Jilin Province. (see ref  2 below)

"Prevalence surveys:
      8.82 percent of forest rodents in Jilin province, 1.64 percent 
in Helongjiang province and 0 percent in Inner Mongolia autonomous 
region (2007 publication)  (see ref 5 below)
      4.6 percent to 6.8 percent  of _I. persulcatus_ in Jilin 
province (2003 to 2005 publications) (see refs 3 and  4 below)
      14.1 percent of rodents captured from a mountainous area in 
southeastern China (Zhejiang Province, 2008 publication) (see ref 6 below)

"Seroprevalence surveys:
      8.8 percent of farm workers near Tianjin (2006) (see ref 7 below)."

In Zhang et al's Rickettsial Seroepidemiology among Farm Workers, 
Tianjin, People's Republic of China. (see ref 7 below), the authors 
conclude that "human infections with these zoonotic bacteria are 
frequent and largely unrecognized." (The zoonotic bacteria and 
seroprevalence mentioned in the study are: _Anaplasma 
phagocytophilum_ (8.8 percent), _Coxiella burnetii_ (6.4 percent), 
_Bartonella henselae_ (9.6 percent), and _Rickettsia typhi_ (4.1 
percent)).  Since the investigation conducted in Shandong was active 
case searching and not a serosurvey of the general population to 
identify subclinical or very mild infections, it is possible that 
there were many such sub-clinical or very mild infections occurring 
which would markedly increase the denominator and thereby lower the 
observed case fatality rate observed in this current incident.

For a map of China with geographic divisions see 
<http://www.lib.utexas.edu/maps/middle_east_and_asia/china_pol01.jpg>. 
Of note, Shandong province is contiguously with Hebei province to the 
north, where Tianjin is located -- one might conclude that a 
seroepidemiology of rural areas in Shandong province would lead to 
similar results as seen in the neighboring province.

The interactive HealthMap/ProMED map of China can be accessed at 
<http://healthmap.org/promed?v=36.5,103.9,4> This map provides links 
to other recent ProMED-mail postings on events occurring in China and 
neighboring countries.

Suggested references:
1.  Chahan B, Jian Z, Xuan X, Sato Y, et al.   Serological evidence 
of infection of Anaplasma and Ehrlichia in domestic animals in 
Xinjiang Uygur Autonomous Region area, China. Vet Parasitol 2005 Dec 
10;134(3-4):273-8.
2.  Cao WC, Zhan L, He J, Foley JE,et al.  Natural Anaplasma 
phagocytophilum infection of ticks and rodents from a forest area of 
Jilin Province, China.  Am J Trop Med Hyg 2006 Oct ;75(4):664-8.
3.  Zhao QM, Wu XM, Zhang PH, Li JM, et al.  [Study on the 
coinfection of three tick-borne infectious diseases in China using 
polymerase chain reaction method]	 Zhonghua Liu Xing Bing Xue Za Zhi 
2005 Jan ;26(1):9-13.
4.  Wen B, Cao W, Pan H.  Ehrlichiae and ehrlichial diseases in 
China.  Ann N Y Acad Sci 2003 Jun ;990():45-53.
5. Zhan L, He J, Saren GW, Wu XM, et al.  [Investigation on Anaplasma 
phagocytophilum infection in rodents from forest areas in 
northeastern China].  Zhonghua Liu Xing Bing Xue Za Zhi 2007 Feb ;28(2):157-9.
6.  Zhan L, Cao WC, De Vlas S, Xie SY, et al.  A Newly Discovered 
Anaplasma phagocytophilum Variant in Rodents from Southeastern 
China.	 Vector Borne Zoonotic Dis 2008 May 9.
7.  Zhang L, Shan A, Mathew B, Yin J, et al.  Rickettsial 
Seroepidemiology among Farm Workers, Tianjin, People's Republic of 
China.  Emerg Infect Dis 2008 Jun ;14(6):938-940. [full article 
available at: <http://www.cdc.gov/eid/content/14/6/pdfs/938.pdf>]

- Mod.MPP]

[see also:
Undiagnosed hemorrhagic disease - China: (Shandong), RFI 20080727.2300
Hantavirus infection - Taiwan ex China (Inner Mongolia) 20080119.0249
Anaplasmosis, human granulocytic - Canada: 1st rep., (AB) 20080731.2352]
...................................mpp/ll/mj/mpp

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