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INFLUENZA PANDEMIC (H1N1) 2009 (16): ARGENTINA, SEQUENCING
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A ProMED-mail post
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ProMED-mail is a program of the
International Society for Infectious Diseases
<http://www.isid.org>
Date Wed 22 Jul 2009
From: Gustavo Palacios <gp2050@columbia.edu>
A team of scientists from the Instituto Nacional de Enfermedades
Infecciosas (INEI) [National Institute of Infectious Diseases], "Dr
Carlos G Malbran" ANLIS [National Administration of Laboratories and
Health Institutes]; the Center for Infection and Immunity at Columbia
University; and Roche 454 Life Sciences is decoding the complete
genomic sequences of influenza pandemic (H1N1) 2009 virus isolates
from patients with severe respiratory disease from the current
outbreak in Argentina.
The announcement came as the worldwide death toll from A (H1N1)
gathered pace, with Argentina logging 165 fatalities -- the 2nd
highest number of swine flu deaths after the United States. Sanger
and pyrosequencing of samples from 2 patients with severe disease
revealed 8 amino acid substitutions with respect to isolates obtained
in other geographic sites. No evidence was found to indicate
emergence of resistance to oseltamivir.
The team plans to completely sequence a minimum of 150 viruses from
nasopharyngeal swabs and cultures over the next 7-10 days. These will
be rapidly shared to engage the efforts and insights of the larger
scientific community.
Both INEI and the Center for Infection and Immunity are members of
the laboratory network of the World Health Organization and the Pan
American Health Organization. Quintiles Transnational Corp is
coordinating sample shipments. Roche Life Sciences is contributing
pyrosequence data. The team is led by Elsa Baumeister at INEI with
support from Daniel Cisterna and Viviana Molina at INEI, Ian Lipkin
and Gustavo Palacios at Columbia, and Michael Egholm and Stephen
Hutchison at Roche 454 Life Sciences.
--
Dra Elsa G Baumeister
Coordinadora del Laboratorio para la identificacion
y caracterizacion del virus de influenza A H1N1 pandemico
Departamento Virologia
INEI-ANLIS "Carlos G. Malbran"
Buenos Aires
Argentina
and
W Ian Lipkin, MD
Director, Northeast Biodefense Center
John Snow Professor of Epidemiology and Director
Center for Infection and Immunity and
WHO Collaborating Centre on Diagnostics, Surveillance and
Immunotherapeutics for Emerging Infectious and Zoonotic Diseases
Professor of Neurology and Pathology
Mailman School of Public Health and
College of Physicians & Surgeons of Columbia University
New York, NY
USA
[It would be useful in assessing the significance of these
observations to know the distribution of the mutations resulting in
amino acid substitutions across the 8 sub-units of the genome and
their relationship to the distribution of non-coding mutations. Were
the same mutations found in each of the 2 patients? - Mod.CP]
[see also:
Influenza pandemic (H1N1) 2009 (14): case count 20090722.259
Influenza pandemic (H1N1) 2009 (13): comments 20090722.2598
Influenza pandemic (H1N1) 2009 (12): antivirals 20090722.2597
Influenza pandemic (H1N1) 2009 (11) vaccine issues 20090722.2595
Influenza pandemic (H1N1) 2009 (10): vaccine 20090720.2577
Influenza pandemic (H1N1) 2009 (09): UK, pig stockman 20090718.2560
Influenza pandemic (H1N1) 2009 (08): pandemic origins 20090718.2559
Influenza pandemic (H1N1) 2009 (07): Argentina, swine, alert 20090718.2557
Influenza pandemic (H1N1) 2009 (06): case reporting 20090717.2553
Influenza pandemic (H1N1) 2009 (05): vaccine 20090716.2540
Influenza pandemic (H1N1) 2009 (04): pandemic origins 20090715.2527
Influenza pandemic (H1N1) 2009 (03): vaccine 20090713.2505
Influenza pandemic (H1N1) 2009 (02): obesity risk factor 20090711.2482
Influenza pandemic (H1N1) 2009 - Viet Nam: patient data 20090708.2450
Influenza A (H1N1) - worldwide (86): official nomenclature 20090706.2430]
...................................cp/mj/lm
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